Blood pH, lactate, and ammonia following repeat sprints in trained and untrained men
Physiological responses to repeat sprints
Keywords:
anaerobic, cycling, fatigue, power, WingateAbstract
Purpose: Examine repeat sprint performance along with blood lactate, pH, and plasma ammonia in untrained and trained men. Methods: Participants performed three 30-second Wingate cycling tests separated by five minutes each. Blood pH and lactate were measured pre-exercise and between each sprint, plasma ammonia was measured pre- and post-exercise. Each subject was classified as NO (no exercise), EO (endurance only), RO (resistance only), or RE (resistance and endurance) based upon exercise history. Results: There were significant effects for group in repeat sprint performance, as measured by peak power (Mean peak power for NO 532.88 ± 35.32; EO 607.37 ± 27.05; RO 829.01 ± 74.68; RE 731.60 ± 48.56; F = 6.75; p < 0.01) and mean power (Mean of mean power for NO 378.62 ± 34.06; EO 501.75 ± 35.31; RO 596.48 ± 45.22; RE 550.36 ± 37.51; F = 6.88; p < 0.01). No significant group differences were found for blood lactate, pH, plasma ammonia values, or fatigue index. Conclusions: Similar fatigue responses regardless of training type and that acute physiological responses to repeat sprints, including buffering of blood pH, lactate, and ammonia are not different between groups with different exercise training history.
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Amann, M. (2011). Central and Peripheral Fatigue: Interaction during Cycling Exercise in Humans. Medicine and science in sports and exercise, 43(11), 2039 – 2045. https://doi.org/10.1249/MSS.0b013e31821f59ab
Babij, P., Matthews, S. M., & Rennie, M. J. (1983). Changes in Blood Ammonia,Lactate and Amino Acids in Relation to Workload During Bicycle Ergometer Exercise in Man. European Journal of Applied Physiology, 50, 405–411.
Bentley, D. J., McNaughton, L. R., Thompson, D., Vleck, V. E., & Batterham, A. M. (2001). Peak power output, the lactate threshold, and time trial performance in cyclists. Medicine and science in sports and exercise, 33(12), 2077–2081. https://doi.org/10.1097/00005768-200112000-00016
Bloom, S. R., Johnson, R. H., Park, D. M., Rennie, M. J., & Sulaiman, W. R. (1976). Differences in the metabolic and hormonal response to exercise between racing cyclists and untrained individuals. The Journal of Physiology, 258(1), 1–18. https://doi.org/10.1113/jphysiol.1976.sp011403
Böning, D., Maassen, N., Lindinger, M. I., & Heigenhauser, G. J. F. (2008). Point:Counterpoint:Lactic acid is/is not the only physicochemical contributor to the acidosis of exercise. Journal of Applied Physiology, 105 (1), 358–361. https://doi.org/10.1152/japplphysiol.00162.2008
Boone, T., Board, R., Astorino, T., Baker, J., Brock, S., Dalleck, L., Goulet, E., Gotshall, R., Hutchison, A., Knight-Maloney, M., Kravitz, L., Laskin, J., Lim, Y. A., Lowery, L., Marks, D., Mermier, C., Robergs, R., Vella, C., Wagner, D., … Oden, G. L. (2011). Development of Wingate Anaerobic Test Norms for Highly-Trained Women. Journal of Exercise Physiology online, 14.
Brooks, G.A., Fahey, T.D., & Baldwin, K.M. (2005). Exercise Physiology: Human Bioenergetics and its applications. McGraw Hill.
Brooks, G. A. (2020). Lactate as a fulcrum of metabolism. Redox biology, 35, 101454. https://doi.org/10.1016/j.redox.2020.101454
Calbet, J. A. L., De Paz, J. A., Garatachea, N., Cabeza De Vaca, S., & Chavarren, J. (2003). Anaerobic energy provision does not limit Wingate exercise performance in endurance-trained cyclists. Journal of Applied Physiology, 94(2), 668–676. https://doi.org/10.1152/japplphysiol.00128.2002
Cheetham, M. E., Boobis, L. H., Brooks, S., & Williams, C. (1986). Human muscle metabolism during sprint running. Journal of applied physiology (Bethesda, Md. : 1985), 61(1), 54–60. https://doi.org/10.1152/jappl.1986.61.1.54
Coggan, A. R., Abduljalil, A. M., Swanson, S. C., Earle, M. S., Farris, J. W., Mendenhall, L. A., & Robitaille, P.-M. (1993). Muscle metabolism during exercise in young and older untrained and endurance-trained men. Journal of Applied Physiology, 75(5), 2125–2133.
Coso, J. Del, Hamouti, N., Aguado-Jimenez, R., & Mora-Rodriguez, R. (2009). Respiratory compensation and blood pH regulation during variable intensity exercise in trained versus untrained subjects. European Journal of Applied Physiology, 107(1), 83–93. https://doi.org/10.1007/s00421-009-1101-y
Dobson, G. P., Yamamoto, E., & Hochachka, P. W. (1986). Phosphofructokinase control in muscle: nature and reversal of pH-dependent ATP inhibition. The American journal of physiology, 250(1 Pt 2), R71–R76. https://doi.org/10.1152/ajpregu.1986.250.1.R71
Edwards, R. H. T., Jones, N. L., Oppenheimer, E. A., Hughes, R. L., & Knill-Jones, R. P. (1969). Interrelation of responses during progressive exercise in trained and untrained subjects. Quarterly Journal of Experimental Physiology and Cognate Medical Sciences, 54(4), 394–403. https://doi.org/10.1113/expphysiol.1969.sp002038
Goodwin, M. L., Harris, J. E., Hernández, A., & Gladden, L. B. (2007). Blood lactate measurements and analysis during exercise: A guide for clinicians. In Journal of Diabetes Science and Technology, 1 (4) 558–569. https://doi.org/10.1177/193229680700100414
Graham, T. E., Turcotte, L. P., Kiens, B., & Richter, E. A. (1995). Training and muscle ammonia and amino acid metabolism in humans during prolonged exercise. Journal of applied physiology (Bethesda, Md. : 1985), 78(2), 725–735. https://doi.org/10.1152/jappl.1995.78.2.725
Itoh, H., & Ohkuwa, T. (1990). Peak blood ammonia and lactate after submaximal, maximal and supramaximal exercise in sprinters and long-distance runners. European Journal of Applied Physiology and Occupational Physiology, 60(4), 271–276. https://doi.org/10.1007/BF00379395
Kato, T., Tsukanaka, A., Harada, T., Kosaka, M., & Matsui, N. (2005). Effect of hypercapnia on changes in blood pH, plasma lactate and ammonia due to exercise. European Journal of Applied Physiology, 95(5–6), 400–408. https://doi.org/10.1007/s00421-005-0046-z
Lindinger, M. I., & Heigenhauser, G. J. F. (2008). Counterpoint: Lactic acid is not the only physicochemical contributor to the acidosis of exercise. Journal of Applied Physiology, 105(1). https://doi.org/10.1152/japplphysiol.00162.2008a
Pescatello, L. S. (2014). ACSM’s Guidelines for Exercise Testing and Prescription (Vol. 8). Philadelphia :Lippincott Williams & Wilkins.
Mutch, B. J. C., & Banister, E. W. (1983). Ammonia metabolism in exercise and fatigue: a review. Medicine & Science in Sports & Exercise, 15, 41–50.
Robergs, R. A., Ghiasvand, F., & Parker, D. (2004). Biochemistry of exercise-induced metabolic acidosis. American journal of physiology. Regulatory, integrative and comparative physiology, 287(3), R502–R516. https://doi.org/10.1152/ajpregu.00114.2004
Robergs, R. A., Ghiasvand, F., & Parker, D. (2006). Reply: The wandering argument favoring a lactic acidosis [2]. In American Journal of Physiology - Regulatory Integrative and Comparative Physiology, 291 (1), R238-R239. https://doi.org/10.1152/ajpregu.00081.2006
Roussel, M., Mattei, J. P., Le Fur, Y., Ghattas, B., Cozzone, P. J., & Bendahan, D. (2003). Metabolic determinants of the onset of acidosis in exercising human muscle: a 31 P-MRS study. Journal of Applied Physiology, 94(3), 1145–1152. https://doi.org/10.1152/japplphysiol.01024.2000
Spencer, M., Bishop, D., Dawson, B., & Goodman, C. (2005). Physiological and metabolic responses of repeated-sprint activities: Specific to field-based team sports. Sports Medicine, 35 (12), 1025–1044. https://doi.org/10.2165/00007256-200535120-00003
Stathis, C. G., Febbraio, M. A., Carey, M. F., & Snow, R. J. (1994). Influence of sprint training on human skeletal muscle purine nucleotide metabolism. Journal of applied physiology (Bethesda, Md. : 1985), 76(4), 1802–1809. https://doi.org/10.1152/jappl.1994.76.4.1802
Trapp, E. G., Chisholm, D. J., & Boutcher, S. H. (2007). Metabolic response of trained and untrained women during high-intensity intermittent cycle exercise. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 293(6), R2370–R2375. https://doi.org/10.1152/ajpregu.00780.2006
Vanuxem, D., Delpierre, S., Barlatier, A., & Vanuxem, P. (1993). Changes in blood ammonia induced by a maximum effort in trained and untrained subjects. Archives of Physiology and Biochemistry, 101(6), 405–409. https://doi.org/10.3109/13813459309047000
Wilkinson, D. J., Smeeton, N. J., & Watt, P. W. (2010). Ammonia metabolism, the brain and fatigue; revisiting the link. Progress in neurobiology, 91(3), 200–219. https://doi.org/10.1016/j.pneurobio.2010.01.012
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